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beta Amyloid Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain beta Amyloid.
N° du produit ABIN5067940

Aperçu rapide pour beta Amyloid Kit ELISA (ABIN5067940)

Antigène

Voir toutes beta Amyloid (Abeta) Kits ELISA
beta Amyloid (Abeta) (Amyloid beta (Abeta))

Épitope

AA 1-40

Reactivité

  • 3
  • 1
  • 1
Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Application

ELISA

Type d'échantillon

Cerebrospinal Fluid, Tissue Homogenate, Tissue Culture Medium
  • Fonction

    The Human beta Amyloid 1-40 (Hu A beta40) ELISA kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for the NH2-terminus of Hu b-amyloid has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known Hu b-amyloid 40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1-40 b-amyloid sequence. This COOH- terminal sequence is created upon cleavage of the analyzed precursor. Bound rabbit antibody is detected by the use of a horseradish peroxidase-labeled anti-rabbit antibody. After washing, horseradish peroxidase-labeled anti-rabbit antibody (enzyme) is added. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Hu A beta40 present in the original specimen.

    Analytical Method

    Quantitative

    Réactivité croisée (Details)

    Calculated cross reactivity: Hu

    Sensibilité

    > 6 pg/mL

    Attributs du produit

    β-Amyloid, aa1-40, Human, ELISA Kit

    Ingrédients

    • * beta Amyloid Standard, 1x1vial
    • Standard Diluent Buffer, 1x60ml
    • Microtiter Plate, 1x96 wells
    • beta Amyloid , Pab 1x6ml
    • IgG (HRP) (100X), 1x125ul
    • HRP Diluent, 1x25ml
    • Wash Buffer (25X), 1x100ml
    • TMB, 1x25ml
    • Stop Solution, 1x25ml
  • Restrictions

    For Research Use only
  • Stock

    4 °C,-80 °C

    Stockage commentaire

    4°C/-70°C
  • Antigène Voir toutes beta Amyloid (Abeta) Kits ELISA

    beta Amyloid (Abeta) (Amyloid beta (Abeta))

    Autre désignation

    beta-Amyloid

    Pathways

    Inflammasome
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