Albumin Kit ELISA
Aperçu rapide pour Albumin Kit ELISA (ABIN5068124)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Sensibilité
- 5 ng/mL
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Attributs du produit
- Albumin, Human, ELISA Kit (Serum Albumin, ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341)
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Ingrédients
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- Microtiter Plate: 1x96 wells
- Control (ng/ml), 1x650ul
- Standard (5ng/ml), 1x650ul
- Standard (10ng/ml), 1x650ul
- Standard (20ng/ml), 1x650ul
- Standard (50ng/ml), 1x650ul
- Standard (100ng/ml), 1x650ul
- HRP Conjugate, 100X, 1x150ul
- Sample Diluent, 20X, 1x10ml
- 0: Wash Solution, 100X, 1x10ml
- 1: TMB Substrate, 1x12ml
- 2: Stop Solution (1% O4), 1x12ml
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Plaque
- Pre-coated
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Protocole
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Principle:
- The Human Albumin ELISA kit is based on the binding of human albumin in samples to two antibodies, one immobilized on the microwells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate (TMB) is added and color is developed by the enzymatic reaction of HRP on the substrate, which is directly proportional to the amount of Albumin present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microwell reader. The concentration of albumin in samples and control is calculated from a curve of standards containing known concentrations of albumin.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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Stockage commentaire
- 4°C
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- Albumin (ALB)
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Autre désignation
- Albumin
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Pathways
- Lipid Metabolism
Antigène Voir toutes Albumin (ALB) Kits ELISA
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