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NRF2 Kit ELISA

NFE2L2 Reactivité: Humain Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
N° du produit ABIN5526730
  • Antigène Voir toutes NRF2 (NFE2L2) Kits ELISA
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Reactivité
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    Human NRF2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical NRF2 binding sequences to semi-quantitatively detect active NRF2 in lysates or nuclear extracts. Only available in North America.
    Type d'échantillon
    Cell Lysate, Nuclear Extract
    Analytical Method
    Semi-Quantitative
    Specificité
    The olionucleotide/antibody pair provided in this kit recognizes human NRF2 in whole lysates and nuclear extracts.
    Attributs du produit
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    Ingrédients
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes NRF2 (NFE2L2) Kits ELISA
    NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))
    Autre désignation
    Nrf2 (NFE2L2 Produits)
    Synonymes
    Nrf2 Kit ELISA, nfe2l2 Kit ELISA, wu:fc15g09 Kit ELISA, wu:fj67e03 Kit ELISA, NF-E2R2 Kit ELISA, NRF2 Kit ELISA, AI194320 Kit ELISA, nuclear factor, erythroid 2-like 2a Kit ELISA, nuclear factor, erythroid 2 like 2 S homeolog Kit ELISA, nuclear factor, erythroid 2 like 2 Kit ELISA, nuclear factor, erythroid derived 2, like 2 Kit ELISA, nuclear factor, erythroid 2-like 2 Kit ELISA, nfe2l2a Kit ELISA, nfe2l2.S Kit ELISA, NFE2L2 Kit ELISA, Nfe2l2 Kit ELISA
    ID gène
    4780
    UniProt
    Q16236
    Pathways
    ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling
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