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Acetyl-CoA Carboxylase alpha Kit ELISA

ACACA Reactivité: Humain, Rat, Souris pSer79 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526733
  • Antigène Voir toutes Acetyl-CoA Carboxylase alpha (ACACA) Kits ELISA
    Acetyl-CoA Carboxylase alpha (ACACA)
    Épitope
    pSer79, total
    Reactivité
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Human Phospho-ATF2 (Thr69/71) and Total ATF2 Cell-Based ELISA Cell-Based ELISA Kit. This assay semi-quantitatively measures ACC1 phosphorylated at Serine-79 as well as total ACC1 in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human, Mouse and Rat ACC1 phosphorylated at site Serine-79 as well as total ACC1.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes Acetyl-CoA Carboxylase alpha (ACACA) Kits ELISA
    Acetyl-CoA Carboxylase alpha (ACACA)
    Autre désignation
    ACC1 (ACACA Produits)
    Synonymes
    ACAC Kit ELISA, ACACAD Kit ELISA, ACC Kit ELISA, ACC1 Kit ELISA, ACCA Kit ELISA, A530025K05Rik Kit ELISA, Acac Kit ELISA, Acc1 Kit ELISA, Gm738 Kit ELISA, AT-ACC1 Kit ELISA, EMB22 Kit ELISA, EMBRYO DEFECTIVE 22 Kit ELISA, GK Kit ELISA, GURKE Kit ELISA, PAS3 Kit ELISA, PASTICCINO 3 Kit ELISA, acetyl-CoA carboxylase 1 Kit ELISA, acc Kit ELISA, fj43d01 Kit ELISA, im:7138837 Kit ELISA, si:ch211-199d18.1 Kit ELISA, wu:fj43d01 Kit ELISA, acetyl-CoA carboxylase alpha Kit ELISA, acetyl-Coenzyme A carboxylase alpha Kit ELISA, acetyl-CoA carboxylase 1 Kit ELISA, ACACA Kit ELISA, Acaca Kit ELISA, ACC1 Kit ELISA, acaca Kit ELISA
    ID gène
    31
    UniProt
    Q13085, Q5SWU9, P11497
    Pathways
    AMPK Signaling, Ribonucleoside Biosynthetic Process, L'effet Warburg
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