IL-25 Kit ELISA

N° du produit ABIN5526765

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IL-25.

Aperçu rapide pour IL-25 Kit ELISA (ABIN5526765)

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 2.39 pg/mL - 6000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit IL-25 Kit ELISA

Seuil minimal de détection: 2.39 pg/mL

Fonction: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-17E.

Analytical Method: Quantitative

Specificité: IL-17E

Ingrédients:

• Plate
• Standard
• Diluent

Matériel non inclus: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: protect your eyes

Stock: 4 °C

Stockage commentaire: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Détail du antigène

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Autre désignation: IL-17E

Sujet: Interleukin 17E (IL-17E), also known as IL-25, is a cytokine that belongs to the IL-17 cytokine family and is secreted by type 2 helper T cells (Th2) and mast cells. It shares the sequence similarity with IL-17. This cytokine can induce NF-κB activation, and stimulate the production of IL-8. Both this cytokine and IL-17B are ligands for the cytokine receptor IL-17RB. Studies in mice suggested that this cytokine may be a proinflammatory cytokine favoring Th2-type immune response. IL-17E induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. This cytokine is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. IL-17E can kill some types of breast cancer cells.

ID gène: 64806

NCBI Accession: NM_022789

UniProt: Q9H293