LIF Kit ELISA

N° du produit ABIN5526767

Kit ELISA LIF Souris, Colorimetric test pour la quantification de Souris LIF.

Aperçu rapide pour LIF Kit ELISA (ABIN5526767)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.77-500 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit LIF Kit ELISA

Seuil minimal de détection: 0.77 pg/mL

Fonction: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse LIF.

Analytical Method: Quantitative

Specificité: LIF

Ingrédients: plate, standard, diluent

Matériel non inclus: pipettes, tubes, reader

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Buffer: 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet: Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts. LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.

NCBI Accession: NM_001039537

UniProt: P09056

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding