MGMT Kit ELISA

N° du produit ABIN5564591

Kit ELISA Humain MGMT, test Colorimetric pour la quantification de Humain MGMT.

Aperçu rapide pour MGMT Kit ELISA (ABIN5564591)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Culture Cells, Plasma, Serum

Détail du produit MGMT Kit ELISA

Marque: AssayMax™

Analytical Method: Quantitative

Matériel non inclus: Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)

Information d'application

Durée du test: 4 h

Plaque: Pre-coated

Calcul des résultats:

• Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
• To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
• Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2

Stock: 4 °C,-20 °C

Détail du antigène

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: MGMT

ID gène: 4255

UniProt: P16455

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus