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ATG3 Kit ELISA

ATG3 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5608575
  • Antigène Voir toutes ATG3 Kits ELISA
    ATG3 (ATG3 Autophagy Related 3 (ATG3))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATG3 in human tissue homogenates, cell lysates and other biological fluids.
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Sensibilité
    6.9 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product ATG3 Kit ELISA
  • Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Procédure de l'essai

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV&lt10%
    • Inter-assay: CV&lt12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes ATG3 Kits ELISA
    ATG3 (ATG3 Autophagy Related 3 (ATG3))
    Autre désignation
    Autophagy Related Protein 3 (ATG3) (ATG3 Produits)
    Synonymes
    atg3 Kit ELISA, im:6910145 Kit ELISA, wu:fb15g12 Kit ELISA, zgc:64156 Kit ELISA, APG3 Kit ELISA, APG3-LIKE Kit ELISA, APG3L Kit ELISA, PC3-96 Kit ELISA, 2610016C12Rik Kit ELISA, Apg3l Kit ELISA, Atg3l Kit ELISA, PIG-1 Kit ELISA, Pig1 Kit ELISA, ATATG3 Kit ELISA, K11J9.1 Kit ELISA, K11J9_1 Kit ELISA, autophagy related 3 Kit ELISA, autophagy 3 (APG3) Kit ELISA, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Kit ELISA, atg3 Kit ELISA, ATG3 Kit ELISA, Atg3 Kit ELISA
    Sujet
    Alternative name: APG3, APG3L, PC3-96, ubiquitin-like-conjugating enzyme ATG3
    ID gène
    64422
    UniProt
    Q9NT62
    Pathways
    Autophagy
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