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ATG3 Kit ELISA

ATG3 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951528
  • Antigène Voir toutes ATG3 Kits ELISA
    ATG3 (ATG3 Autophagy Related 3 (ATG3))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human ATG3 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ATG3 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes ATG3 Kits ELISA
    ATG3 (ATG3 Autophagy Related 3 (ATG3))
    Autre désignation
    ATG3 (ATG3 Produits)
    Synonymes
    atg3 Kit ELISA, im:6910145 Kit ELISA, wu:fb15g12 Kit ELISA, zgc:64156 Kit ELISA, APG3 Kit ELISA, APG3-LIKE Kit ELISA, APG3L Kit ELISA, PC3-96 Kit ELISA, 2610016C12Rik Kit ELISA, Apg3l Kit ELISA, Atg3l Kit ELISA, PIG-1 Kit ELISA, Pig1 Kit ELISA, ATATG3 Kit ELISA, K11J9.1 Kit ELISA, K11J9_1 Kit ELISA, autophagy related 3 Kit ELISA, autophagy 3 (APG3) Kit ELISA, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Kit ELISA, atg3 Kit ELISA, ATG3 Kit ELISA, Atg3 Kit ELISA
    ID gène
    64422
    UniProt
    Q9NT62
    Pathways
    Autophagy
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