Serotonin Receptor 1A Kit ELISA

N° du produit ABIN5651286

Détail du produit Serotonin Receptor 1A Kit ELISA

Antigène: Serotonin Receptor 1A (HTR1A)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 1A (HTR1A). No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 1A (HTR1A) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 1A (HTR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 1A (HTR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 1A (HTR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 1A (HTR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 1A (HTR1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 1A (HTR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Serotonin Receptor 1A (HTR1A)

Autre désignation: 5-Hydroxytryptamine Receptor 1A (HTR1A Produits)

Synonymes: 5-HT-dro2A Kit ELISA, 5-HT1ADro Kit ELISA, 5-HT[1A]Dro Kit ELISA, 5-HT[[1ADro]] Kit ELISA, 5-HT[[1A]] Kit ELISA, 5-HT[[1A]]DRO Kit ELISA, 5HT-R2A Kit ELISA, 5HT-dro2A Kit ELISA, 5HT-drp[[2A]] Kit ELISA, 5HT1A Kit ELISA, 5HT[[1ADro]] Kit ELISA, 5htdro2a Kit ELISA, CG16720 Kit ELISA, DRO2A Kit ELISA, Dm5HTdro2A Kit ELISA, Dmel\\CG16720 Kit ELISA, d5-HT1A Kit ELISA, 5htr1a Kit ELISA, htr1a Kit ELISA, HTR1A Kit ELISA, Htr1a Kit ELISA, 5-Ht1a Kit ELISA, Gpcr18 Kit ELISA, 5-HT-1A Kit ELISA, 5-HT1A Kit ELISA, 5HT1a Kit ELISA, ADRB2RL1 Kit ELISA, ADRBRL1 Kit ELISA, G-21 Kit ELISA, PFMCD Kit ELISA, RAT5HT1A Kit ELISA, 5ht1a Kit ELISA, adrb2rl1 Kit ELISA, adrbrl1 Kit ELISA, htr1a-A Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 1A Kit ELISA, 5-hydroxytryptamine receptor 1A Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 1A a Kit ELISA, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled L homeolog Kit ELISA, 5-HT1A Kit ELISA, HTR1A Kit ELISA, htr1aa Kit ELISA, Htr1a Kit ELISA, htr1a.L Kit ELISA

Sujet:

Gene Name: 5-Hydroxytryptamine Receptor 1A

Gene Aliases: 5HTR1A, 5HTR1-A, 5-HTR1-A, ADRB2RL1, ADRBRL1, Serotonin Receptor 1A

Pathways: Signalistation JAK/STAT, Synaptic Membrane, Feeding Behaviour