ADAM10 Kit ELISA

N° du produit ABIN5651303

Détail du produit ADAM10 Kit ELISA

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 9.4 pg/mL - 600 pg/mL

Seuil minimal de détection: 9.4 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.

Sensibilité: 3.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 10 (ADAM10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 10 (ADAM10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Autre désignation: A Disintegrin And Metalloprotease 10 (ADAM10 Produits)

Synonymes: ADAM10 Kit ELISA, Adam10 Kit ELISA, adam10 Kit ELISA, wu:fc03d12 Kit ELISA, ad10 Kit ELISA, cd156c Kit ELISA, kuz Kit ELISA, kuzbanian Kit ELISA, madm Kit ELISA, xadam10 Kit ELISA, ADAM-10 Kit ELISA, ADAM 10 Kit ELISA, AD10 Kit ELISA, CD156c Kit ELISA, HsT18717 Kit ELISA, MADM Kit ELISA, 1700031C13Rik Kit ELISA, LOC100219653 Kit ELISA, zgc:64203 Kit ELISA, ADAM metallopeptidase domain 10 Kit ELISA, ADAM metallopeptidase domain 10a Kit ELISA, ADAM metallopeptidase domain 10 S homeolog Kit ELISA, a disintegrin and metallopeptidase domain 10 Kit ELISA, ADAM metallopeptidase domain 10b Kit ELISA, ADAM10 Kit ELISA, Adam10 Kit ELISA, adam10a Kit ELISA, adam10.S Kit ELISA, adam10b Kit ELISA

Sujet:

Gene Name: A Disintegrin And Metalloprotease 10

Gene Aliases: CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

Pathways: Signalisation Notch, EGFR Signaling Pathway