beta Actin Kit CLIA

N° du produit ABIN5651364

Détail du produit beta Actin Kit CLIA

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Rat, Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Sensibilité: 0.05 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Actin Beta (ACTb) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: Actin Beta (ACTB Produits)

Synonymes: BRWS1 Kit CLIA, PS1TP5BP1 Kit CLIA, ACTA1 Kit CLIA, Actx Kit CLIA, E430023M04Rik Kit CLIA, beta-actin Kit CLIA, ps1tp5bp1 Kit CLIA, act-2 Kit CLIA, actb Kit CLIA, B-actin Kit CLIA, LOC100174883 Kit CLIA, LOC100304816 Kit CLIA, A Kit CLIA, ACT5C Kit CLIA, Ac5C Kit CLIA, Act Kit CLIA, Act-5C Kit CLIA, Act5 Kit CLIA, Act5c Kit CLIA, Actin Kit CLIA, Actin/BAP47 Kit CLIA, Actin5C Kit CLIA, BAP47 Kit CLIA, Bap47 Kit CLIA, CG4027 Kit CLIA, Dmel\\CG4027 Kit CLIA, M32055 Kit CLIA, T11 Kit CLIA, act Kit CLIA, act 5C Kit CLIA, act5C Kit CLIA, actin Kit CLIA, actin5C Kit CLIA, anon-EST:fe2D2 Kit CLIA, beta-actin/Bap47 Kit CLIA, cyt5C Kit CLIA, l(1)G0009 Kit CLIA, l(1)G0010 Kit CLIA, l(1)G0025 Kit CLIA, l(1)G0079 Kit CLIA, l(1)G0117 Kit CLIA, l(1)G0177 Kit CLIA, l(1)G0245 Kit CLIA, l(1)G0330 Kit CLIA, l(1)G0420 Kit CLIA, l(1)G0486 Kit CLIA, Bact Kit CLIA, ACT-5 Kit CLIA, ACTB Kit CLIA, B-ACTZF Kit CLIA, actba Kit CLIA, bact Kit CLIA, bactin1 Kit CLIA, bactzf Kit CLIA, wu:fd18f05 Kit CLIA, A26C1B Kit CLIA, POTE2alpha Kit CLIA, POTEACTIN Kit CLIA, Beta-actin Kit CLIA, actin beta Kit CLIA, actin, beta Kit CLIA, cytoplasmic actin OlCA1 Kit CLIA, beta-actin Kit CLIA, Actin 5C Kit CLIA, actin gamma 1 Kit CLIA, actin, beta 1 Kit CLIA, actin, beta L homeolog Kit CLIA, beta actin Kit CLIA, POTE ankyrin domain family member F Kit CLIA, actin, beta like 2 Kit CLIA, actin, cytoplasmic 1 Kit CLIA, ACTB Kit CLIA, Actb Kit CLIA, actb Kit CLIA, LOC100174883 Kit CLIA, LOC100304816 Kit CLIA, Act5C Kit CLIA, ACTG1 Kit CLIA, actb1 Kit CLIA, actb.L Kit CLIA, LOC100136352 Kit CLIA, POTEF Kit CLIA, ACTBL2 Kit CLIA, LOC109073280 Kit CLIA

Sujet:

Gene Name: Actin Beta

Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction