beta Actin Kit ELISA

N° du produit ABIN5651365

Détail du produit beta Actin Kit ELISA

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Sensibilité: 0.57 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Beta (ACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Beta (ACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: Actin Beta (ACTB Produits)

Synonymes: BRWS1 Kit ELISA, PS1TP5BP1 Kit ELISA, ACTA1 Kit ELISA, Actx Kit ELISA, E430023M04Rik Kit ELISA, beta-actin Kit ELISA, ps1tp5bp1 Kit ELISA, act-2 Kit ELISA, actb Kit ELISA, B-actin Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, A Kit ELISA, ACT5C Kit ELISA, Ac5C Kit ELISA, Act Kit ELISA, Act-5C Kit ELISA, Act5 Kit ELISA, Act5c Kit ELISA, Actin Kit ELISA, Actin/BAP47 Kit ELISA, Actin5C Kit ELISA, BAP47 Kit ELISA, Bap47 Kit ELISA, CG4027 Kit ELISA, Dmel\\CG4027 Kit ELISA, M32055 Kit ELISA, T11 Kit ELISA, act Kit ELISA, act 5C Kit ELISA, act5C Kit ELISA, actin Kit ELISA, actin5C Kit ELISA, anon-EST:fe2D2 Kit ELISA, beta-actin/Bap47 Kit ELISA, cyt5C Kit ELISA, l(1)G0009 Kit ELISA, l(1)G0010 Kit ELISA, l(1)G0025 Kit ELISA, l(1)G0079 Kit ELISA, l(1)G0117 Kit ELISA, l(1)G0177 Kit ELISA, l(1)G0245 Kit ELISA, l(1)G0330 Kit ELISA, l(1)G0420 Kit ELISA, l(1)G0486 Kit ELISA, Bact Kit ELISA, ACT-5 Kit ELISA, ACTB Kit ELISA, B-ACTZF Kit ELISA, actba Kit ELISA, bact Kit ELISA, bactin1 Kit ELISA, bactzf Kit ELISA, wu:fd18f05 Kit ELISA, A26C1B Kit ELISA, POTE2alpha Kit ELISA, POTEACTIN Kit ELISA, Beta-actin Kit ELISA, actin beta Kit ELISA, actin, beta Kit ELISA, cytoplasmic actin OlCA1 Kit ELISA, beta-actin Kit ELISA, Actin 5C Kit ELISA, actin gamma 1 Kit ELISA, actin, beta 1 Kit ELISA, actin, beta L homeolog Kit ELISA, beta actin Kit ELISA, POTE ankyrin domain family member F Kit ELISA, actin, beta like 2 Kit ELISA, actin, cytoplasmic 1 Kit ELISA, ACTB Kit ELISA, Actb Kit ELISA, actb Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, Act5C Kit ELISA, ACTG1 Kit ELISA, actb1 Kit ELISA, actb.L Kit ELISA, LOC100136352 Kit ELISA, POTEF Kit ELISA, ACTBL2 Kit ELISA, LOC109073280 Kit ELISA

Sujet:

Gene Name: Actin Beta

Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction