ADRB3 Kit ELISA
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- Antigène Voir toutes ADRB3 Kits ELISA
- ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.62 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.62 pg/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Adrenergic Receptor Beta 3 (ADRb3). No significant cross-reactivity or interference between Adrenergic Receptor Beta 3 (ADRb3) and analogues was observed.
- Sensibilité
- 5.9 pg/mL
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- Discover our top product ADRB3 Kit ELISA
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adrenergic Receptor Beta 3 (ADRb3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adrenergic Receptor Beta 3 (ADRb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adrenergic Receptor Beta 3 (ADRb3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenergic Receptor Beta 3 (ADRb3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes ADRB3 Kits ELISA
- ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))
- Autre désignation
- Adrenergic Receptor Beta 3 (ADRB3 Produits)
- Synonymes
- BETA3AR Kit ELISA, Adrb-3 Kit ELISA, ADRB Kit ELISA, BAR3 Kit ELISA, B3AR Kit ELISA, GPCR Kit ELISA, im:7154935 Kit ELISA, si:ch211-215f19.1 Kit ELISA, zgc:194843 Kit ELISA, zgc:194852 Kit ELISA, adrenoceptor beta 3 Kit ELISA, adrenergic receptor, beta 3 Kit ELISA, adrenoceptor beta 3a Kit ELISA, adrenoceptor beta 3b Kit ELISA, ADRB3 Kit ELISA, Adrb3 Kit ELISA, adrb3a Kit ELISA, adrb3b Kit ELISA
- Sujet
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Gene Name: Adrenergic Receptor Beta 3
Gene Aliases: BETA3AR, B3AR, Beta-3 adrenoreceptor
- Pathways
- cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation
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