Intestinal Alkaline Phosphatase Kit ELISA

N° du produit ABIN5651582

Détail du produit Intestinal Alkaline Phosphatase Kit ELISA

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Intestinal (ALPI). No significant cross-reactivity or interference between Alkaline Phosphatase, Intestinal (ALPI) and analogues was observed.

Sensibilité: 0.65 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Intestinal (ALPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Intestinal (ALPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Intestinal (ALPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alkaline Phosphatase, Intestinal (ALPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Autre désignation: Alkaline Phosphatase, Intestinal (ALPI Produits)

Synonymes: IAP Kit ELISA, Alp1 Kit ELISA, S51097 Kit ELISA, 2010001C14Rik Kit ELISA, Akp6 Kit ELISA, gIAP Kit ELISA, alpi Kit ELISA, im:7149588 Kit ELISA, si:ch211-262h13.1 Kit ELISA, wu:fa95f04 Kit ELISA, zgc:110794 Kit ELISA, ALPI Kit ELISA, Alpi Kit ELISA, alkaline phosphatase, intestinal Kit ELISA, alkaline phosphatase, intestinal, tandem duplicate 1 Kit ELISA, intestinal-type alkaline phosphatase Kit ELISA, ALPI Kit ELISA, Alpi Kit ELISA, alpi.1 Kit ELISA, LOC100724954 Kit ELISA

Sujet:

Gene Name: Alkaline Phosphatase, Intestinal

Gene Aliases: CIP, IAP, Calf Intestinal Alkaline Phosphatase

ID gène: 248

UniProt: P09923