alpha 2 Macroglobulin Kit CLIA

N° du produit ABIN5651629

Détail du produit alpha 2 Macroglobulin Kit CLIA

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.39 ng/mL - 100 ng/mL

Seuil minimal de détection: 0.39 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Urine

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.

Sensibilité: 0.05 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Alpha-2-Macroglobulin (a2M) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: Alpha-2-Macroglobulin (A2M Produits)

Synonymes: A2MD Kit CLIA, CPAMD5 Kit CLIA, FWP007 Kit CLIA, S863-7 Kit CLIA, A2MAC1 Kit CLIA, A2m1 Kit CLIA, A2maa Kit CLIA, Mam Kit CLIA, A2mp Kit CLIA, A2M Kit CLIA, LOC733429 Kit CLIA, endod Kit CLIA, cpamd5 Kit CLIA, fwp007 Kit CLIA, s863-7 Kit CLIA, a2mb Kit CLIA, endodermin Kit CLIA, Alpha-2-M Kit CLIA, alpha-2-macroglobulin Kit CLIA, pregnancy-zone protein L homeolog Kit CLIA, alpha-2-macroglobulin S homeolog Kit CLIA, pregnancy zone protein Kit CLIA, alpha-2-macroglobulin-like Kit CLIA, A2M Kit CLIA, A2m Kit CLIA, LOC477699 Kit CLIA, pzp.L Kit CLIA, AZL_c00450 Kit CLIA, LOC100349077 Kit CLIA, a2m Kit CLIA, LOC100061656 Kit CLIA, LOC100090399 Kit CLIA, a2m.S Kit CLIA, LOC100469973 Kit CLIA, LOC100595735 Kit CLIA, PZP Kit CLIA, LOC100353095 Kit CLIA, LOC101122940 Kit CLIA, LOC101801552 Kit CLIA, LOC100911545 Kit CLIA

Sujet:

Gene Name: Alpha-2-Macroglobulin

Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

ID gène: 2

UniProt: P01023

Pathways: Lipid Metabolism