alpha 2 Macroglobulin Kit ELISA

N° du produit ABIN5651632

Détail du produit alpha 2 Macroglobulin Kit ELISA

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 12.5 ng/mL - 800 ng/mL

Seuil minimal de détection: 12.5 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Urine

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Alpha-2-Macroglobulin (a2M). No significant cross-reactivity or interference between Alpha-2-Macroglobulin (a2M) and analogues was observed.

Sensibilité: 5.9 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin (a2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin (a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin (a2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin (a2M) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Macroglobulin (a2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: Alpha-2-Macroglobulin (A2M Produits)

Synonymes: A2MD Kit ELISA, CPAMD5 Kit ELISA, FWP007 Kit ELISA, S863-7 Kit ELISA, A2MAC1 Kit ELISA, A2m1 Kit ELISA, A2maa Kit ELISA, Mam Kit ELISA, A2mp Kit ELISA, A2M Kit ELISA, LOC733429 Kit ELISA, endod Kit ELISA, cpamd5 Kit ELISA, fwp007 Kit ELISA, s863-7 Kit ELISA, a2mb Kit ELISA, endodermin Kit ELISA, Alpha-2-M Kit ELISA, alpha-2-macroglobulin Kit ELISA, pregnancy-zone protein L homeolog Kit ELISA, alpha-2-macroglobulin S homeolog Kit ELISA, pregnancy zone protein Kit ELISA, alpha-2-macroglobulin-like Kit ELISA, A2M Kit ELISA, A2m Kit ELISA, LOC477699 Kit ELISA, pzp.L Kit ELISA, AZL_c00450 Kit ELISA, LOC100349077 Kit ELISA, a2m Kit ELISA, LOC100061656 Kit ELISA, LOC100090399 Kit ELISA, a2m.S Kit ELISA, LOC100469973 Kit ELISA, LOC100595735 Kit ELISA, PZP Kit ELISA, LOC100353095 Kit ELISA, LOC101122940 Kit ELISA, LOC101801552 Kit ELISA, LOC100911545 Kit ELISA

Sujet:

Gene Name: Alpha-2-Macroglobulin

Gene Aliases: A2MG, A2 mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

ID gène: 100911545

UniProt: P06238

Pathways: Lipid Metabolism