Angiopoietin 2 Kit CLIA

N° du produit ABIN5651738

Détail du produit Angiopoietin 2 Kit CLIA

Antigène: Angiopoietin 2 (ANGPT2)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 6.9 pg/mL - 5000 pg/mL

Seuil minimal de détection: 6.9 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Angiopoietin 2 (ANGPT2). No significant cross-reactivity or interference between Angiopoietin 2 (ANGPT2) and analogues was observed.

Sensibilité: 2.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Angiopoietin 2 (ANGPT2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiopoietin 2 (ANGPT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiopoietin 2 (ANGPT2) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiopoietin 2 (ANGPT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Angiopoietin 2 (ANGPT2)

Autre désignation: Angiopoietin 2 (ANGPT2 Produits)

Synonymes: AGPT2 Kit CLIA, ANG2 Kit CLIA, Agpt2 Kit CLIA, Ang-2 Kit CLIA, Ang2 Kit CLIA, ang2 Kit CLIA, angpt2 Kit CLIA, ANGIOPOIETIN-2 Kit CLIA, ANG-2 Kit CLIA, ANGPT2 Kit CLIA, Angrp Kit CLIA, Rnase5b Kit CLIA, ANG3 Kit CLIA, C11orf2 Kit CLIA, C11orf3 Kit CLIA, FFR Kit CLIA, angiopoietin 2 Kit CLIA, angiopoietin 2b Kit CLIA, angiogenin, ribonuclease A family, member 2 Kit CLIA, VPS51, GARP complex subunit Kit CLIA, ANGPT2 Kit CLIA, Angpt2 Kit CLIA, angpt2b Kit CLIA, Ang2 Kit CLIA, VPS51 Kit CLIA

Sujet:

Gene Name: Angiopoietin 2

Gene Aliases: ANG2, AGPT2

ID gène: 285

UniProt: O15123

Pathways: Signalisation RTK