APOA1 Kit CLIA
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- Antigène Voir toutes APOA1 Kits CLIA
- APOA1 (Apolipoprotein A-I (APOA1))
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Reactivité
- Souris
- Méthode de détection
- Chemiluminescent
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.39 ng/mL - 100 ng/mL
- Seuil minimal de détection
- 0.39 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
- Sensibilité
- 0.07 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 - 3 h
- Plaque
- Pre-coated
- Protocole
- The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein A1 (APOA1) level in the sample or standard.,
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes APOA1 Kits CLIA
- APOA1 (Apolipoprotein A-I (APOA1))
- Autre désignation
- Apolipoprotein A1 (APOA1 Produits)
- Synonymes
- Alp-1 Kit CLIA, Apoa-1 Kit CLIA, Brp-14 Kit CLIA, Ltw-1 Kit CLIA, Lvtw-1 Kit CLIA, Sep-1 Kit CLIA, Sep-2 Kit CLIA, Sep2 Kit CLIA, apoA-I Kit CLIA, apoa Kit CLIA, apoa1 Kit CLIA, cb49 Kit CLIA, wu:fb33f01 Kit CLIA, zgc:103718 Kit CLIA, MGC64335 Kit CLIA, MGC89745 Kit CLIA, Apo-AI Kit CLIA, ApoA-I Kit CLIA, APOA1 Kit CLIA, LOC100136573 Kit CLIA, apoa-i Kit CLIA, ApoAI Kit CLIA, apolipoprotein A1 Kit CLIA, apolipoprotein A-I Kit CLIA, apolipoprotein A-Ia Kit CLIA, apolipoprotein A-I S homeolog Kit CLIA, apolipoprotein A4 Kit CLIA, APOA1 Kit CLIA, Apoa1 Kit CLIA, apoa1a Kit CLIA, apoa1.S Kit CLIA, apoa1 Kit CLIA, LOC100136573 Kit CLIA, apoa-i Kit CLIA, apoa4 Kit CLIA
- Sujet
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Gene Name: Apolipoprotein A1
Gene Aliases: Apo-A1, ApoA-1 Milano, ProapoA-I, Proapolipoprotein A-I, Truncated apolipoprotein A-I
- ID gène
- 11806
- UniProt
- Q00623
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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