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APOA2 Kit ELISA

APOA2 Reactivité: Rat Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
N° du produit ABIN5651896
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    APOA2 (Apolipoprotein A-II (APOA2))
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    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    6.25 ng/mL - 400 ng/mL
    Seuil minimal de détection
    6.25 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A2 (APOA2). No significant cross-reactivity or interference between Apolipoprotein A2 (APOA2) and analogues was observed.
    Sensibilité
    2.57 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A2 (APOA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A2 (APOA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A2 (APOA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A2 (APOA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes APOA2 Kits ELISA
    APOA2 (Apolipoprotein A-II (APOA2))
    Autre désignation
    Apolipoprotein A2 (APOA2 Produits)
    Synonymes
    Apo-AII Kit ELISA, ApoA-II Kit ELISA, apoAII Kit ELISA, Alp-2 Kit ELISA, ApoAII Kit ELISA, Apoa-2 Kit ELISA, Hdl-1 Kit ELISA, APOAII Kit ELISA, apoa2 Kit ELISA, apoA-II Kit ELISA, cb1032 Kit ELISA, wu:fb57h11 Kit ELISA, zgc:193613 Kit ELISA, apolipoprotein A2 Kit ELISA, apolipoprotein A-II Kit ELISA, APOA2 Kit ELISA, Apoa2 Kit ELISA, apoa2 Kit ELISA
    Sujet

    Gene Name: Apolipoprotein A2

    Gene Aliases: Apo-A2, Apolipoprotein A-II, ProapoA-II, Truncated apolipoprotein A-II

    ID gène
    25649
    UniProt
    P04638
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism
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