APOE Kit ELISA
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- Antigène Voir toutes APOE Kits ELISA
- APOE (Apolipoprotein E (APOE))
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Reactivité
- Lapin
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 493.83 pg/mL - 40000 pg/mL
- Seuil minimal de détection
- 493.83 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Apolipoprotein E (APOE). No significant cross-reactivity or interference between Apolipoprotein E (APOE) and analogues was observed.
- Sensibilité
- 182.5 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 2 h
- Plaque
- Pre-coated
- Protocole
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apolipoprotein E (APOE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apolipoprotein E (APOE) and unlabeled Apolipoprotein E (APOE) (Standards or samples) with the pre-coated antibody specific to Apolipoprotein E (APOE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apolipoprotein E (APOE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apolipoprotein E (APOE) in the sample.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes APOE Kits ELISA
- APOE (Apolipoprotein E (APOE))
- Autre désignation
- Apolipoprotein E (APOE Produits)
- Synonymes
- ad2 Kit ELISA, apoprotein Kit ELISA, im:7036787 Kit ELISA, wu:fb69a05 Kit ELISA, zgc:110064 Kit ELISA, apoe Kit ELISA, AI255918 Kit ELISA, AD2 Kit ELISA, LDLCQ5 Kit ELISA, LPG Kit ELISA, APOEA Kit ELISA, Apo-E Kit ELISA, apolipoprotein E Kit ELISA, apolipoprotein Ea Kit ELISA, apoe Kit ELISA, apoea Kit ELISA, Apoe Kit ELISA, APOE Kit ELISA
- Sujet
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Gene Name: Apolipoprotein E
Gene Aliases: Apo-E, AD2, Apoprotein, Alzheimer Disease 2(E4-Associated,Late Onset
- Pathways
- Regulation of Cell Size, Lipid Metabolism
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