Asialoglycoprotein Receptor 2 Kit ELISA (ASGR2)

Details for Product ASGR2 ELISA Kit No. ABIN5652039
Antigène
  • ASGR2
  • asgr2
  • MGC137129
  • ASGP-R2
  • ASGPR2
  • CLEC4H2
  • HBXBP
  • HL-2
  • Asgr
  • Asgr-2
  • asialoglycoprotein receptor 2
  • C-type lectin domain family 10 member A
  • ASGR2
  • clec10a
  • Asgr2
  • LOC100347178
Reactivité
Humain
Alternatives
Kits with alternative reactivity to:
11
3
2
2
2
2
2
1
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
62.5 pg/mL - 4000 pg/mL
Seuil minimal de détection
62.5 pg/mL
Application
ELISA
Options
Type d'échantillon Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This assay has high sensitivity and excellent specificity for detection of Asialoglycoprotein Receptor 2 (ASGR2). No significant cross-reactivity or interference between Asialoglycoprotein Receptor 2 (ASGR2) and analogues was observed.
Sensibilité 27.1 pg/mL
Plasmids, Primers & others Plasmids, Primers & others Asialoglycoprotein Receptor 2 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Asialoglycoprotein Receptor 2 (ASGR2 ELISA Kit Extrait)
Sujet

Gene Name: Asialoglycoprotein Receptor 2

Gene Aliases: CLEC4H2, Asialoglyco Protein Receptor 2, C-type lectin domain family 4 member H2, Hepatic lectin H2

ID gène 433
UniProt P07307
Pathways Thyroid Hormone Synthesis
Commentaires

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test 3 h
Plaque Pre-coated
Protocole The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asialoglycoprotein Receptor 2 (ASGR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asialoglycoprotein Receptor 2 (ASGR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asialoglycoprotein Receptor 2 (ASGR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asialoglycoprotein Receptor 2 (ASGR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Précision du teste Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asialoglycoprotein Receptor 2 (ASGR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asialoglycoprotein Receptor 2 (ASGR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Conseil sur la manipulation The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Stock 4 °C,-20 °C
Stockage commentaire -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Date de péremption 4-8 months
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