Asparagine Synthetase Kit ELISA

N° du produit ABIN5652040

Détail du produit Asparagine Synthetase Kit ELISA

Antigène: Asparagine Synthetase (ASNS)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Asparagine Synthetase (ASNS). No significant cross-reactivity or interference between Asparagine Synthetase (ASNS) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Asparagine Synthetase (ASNS)

Autre désignation: Asparagine Synthetase (ASNS Produits)

Synonymes: zgc:56605 Kit ELISA, zgc:76901 Kit ELISA, LOC692995 Kit ELISA, ASNS Kit ELISA, GB13219 Kit ELISA, CG33486 Kit ELISA, Dmel\\CG33486 Kit ELISA, asns Kit ELISA, TS11 Kit ELISA, ARABIDOPSIS THALIANA GLUTAMINE-DEPENDENT ASPARAGINE SYNTHASE 1 Kit ELISA, AT-ASN1 Kit ELISA, DARK INDUCIBLE 6 Kit ELISA, DIN6 Kit ELISA, GLUTAMINE-DEPENDENT ASPARAGINE SYNTHETASE Kit ELISA, glutamine-dependent asparagine synthase 1 Kit ELISA, AS Kit ELISA, AsnS1 Kit ELISA, asparagine synthetase Kit ELISA, asparagine synthetase (glutamine-hydrolyzing) Kit ELISA, asparagine synthetase [glutamine-hydrolyzing] Kit ELISA, Asparagine synthetase Kit ELISA, glutamine-dependent asparagine synthase 1 Kit ELISA, asparagine synthetase homolog 1 Kit ELISA, asns Kit ELISA, ASNS Kit ELISA, LOC692995 Kit ELISA, LOC724480 Kit ELISA, asnB Kit ELISA, AsnS Kit ELISA, Asns Kit ELISA, ASN1 Kit ELISA, asn1 Kit ELISA

Sujet:

Gene Name: Asparagine Synthetase

Gene Aliases: Aspartate-Ammonia Ligase, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

ID gène: 440

UniProt: P08243

Pathways: ER-Nucleus Signaling