Beclin 1 Kit ELISA

N° du produit ABIN5652173

Détail du produit Beclin 1 Kit ELISA

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Beclin 1 (BECN1). No significant cross-reactivity or interference between Beclin 1 (BECN1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beclin 1 (BECN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beclin 1 (BECN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beclin 1 (BECN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beclin 1 (BECN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beclin 1 (BECN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beclin 1 (BECN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Autre désignation: Beclin 1 (BECN1 Produits)

Synonymes: beclin-1 Kit ELISA, atg6 Kit ELISA, vps30 Kit ELISA, beclin1 Kit ELISA, Beclin-1 Kit ELISA, becn1 Kit ELISA, zgc:73097 Kit ELISA, ATG6 Kit ELISA, VPS30 Kit ELISA, 4921513J16Rik Kit ELISA, 5430417M23Rik Kit ELISA, Atg6 Kit ELISA, beclin 1 Kit ELISA, beclin 1, autophagy related Kit ELISA, beclin 1 protein Kit ELISA, beclin-1 Kit ELISA, beclin family protein Kit ELISA, beclin 1, autophagy related L homeolog Kit ELISA, BECN1 Kit ELISA, becn1 Kit ELISA, atg6 Kit ELISA, CpipJ_CPIJ011087 Kit ELISA, PTRG_07825 Kit ELISA, SJAG_01876 Kit ELISA, Becn1 Kit ELISA, becn1.L Kit ELISA

Sujet:

Gene Name: Beclin 1

Gene Aliases: ATG6, VPS30, Coiled-Coil,Myosin-Like BCL2 Interacting Protein, ATG6 Autophagy Related 6 Homolog

ID gène: 8678

UniProt: Q14457

Pathways: Autophagy