BID Kit ELISA

N° du produit ABIN5652220

Détail du produit BID Kit ELISA

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of BH3 Interacting Domain Death Agonist (Bid). No significant cross-reactivity or interference between BH3 Interacting Domain Death Agonist (Bid) and analogues was observed.

Sensibilité: 0.067 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BH3 Interacting Domain Death Agonist (Bid). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BH3 Interacting Domain Death Agonist (Bid). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BH3 Interacting Domain Death Agonist (Bid), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BH3 Interacting Domain Death Agonist (Bid) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BH3 Interacting Domain Death Agonist (Bid) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BH3 Interacting Domain Death Agonist (Bid) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Autre désignation: BH3 Interacting Domain Death Agonist (BID Produits)

Synonymes: FP497 Kit ELISA, 2700049M22Rik Kit ELISA, AI875481 Kit ELISA, AU022477 Kit ELISA, BID Kit ELISA, bid Kit ELISA, si:ch211-238n5.6 Kit ELISA, fp497 Kit ELISA, xbid Kit ELISA, DKFZp469E066 Kit ELISA, BH3 interacting domain death agonist Kit ELISA, BH3 interacting domain death agonist S homeolog Kit ELISA, BID Kit ELISA, Bid Kit ELISA, bida Kit ELISA, bid.S Kit ELISA, bid Kit ELISA

Sujet:

Gene Name: BH3 Interacting Domain Death Agonist

Gene Aliases: p22 BID

ID gène: 64625

UniProt: Q9JLT6

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity