BMP7 Kit ELISA

N° du produit ABIN5652291

Détail du produit BMP7 Kit ELISA

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 7 (BMP7). No significant cross-reactivity or interference between Bone Morphogenetic Protein 7 (BMP7) and analogues was observed.

Sensibilité: 5.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 7 (BMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 7 (BMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 7 (BMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 7 (BMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 7 (BMP7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 7 (BMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: Bone Morphogenetic Protein 7 (BMP7 Produits)

Synonymes: bmp-7 Kit ELISA, bmp7 Kit ELISA, op-1 Kit ELISA, BMP7 Kit ELISA, zgc:153025 Kit ELISA, OP-1 Kit ELISA, OP1 Kit ELISA, BMP-7 Kit ELISA, fb83f09 Kit ELISA, wu:fb83f09 Kit ELISA, bone morphogenetic protein 7 Kit ELISA, bone morphogenetic protein 7, gene 1 L homeolog Kit ELISA, bone morphogenetic protein 7b Kit ELISA, bone morphogenetic protein 7, gene 2 L homeolog Kit ELISA, bone morphogenetic protein 7a Kit ELISA, BMP7 Kit ELISA, bmp7.1.L Kit ELISA, LOC100136282 Kit ELISA, bmp7b Kit ELISA, Bmp7 Kit ELISA, LOC100008826 Kit ELISA, bmp7.2.L Kit ELISA, bmp7a Kit ELISA

Sujet:

Gene Name: Bone Morphogenetic Protein 7

Gene Aliases: OP1, Osteogenic Protein-1, Eptotermin alfa

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance