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CTNNB1 Kit ELISA

CTNNB1 Reactivité: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652681
  • Antigène Voir toutes CTNNB1 Kits ELISA
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Reactivité
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1). No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
    Sensibilité
    13.4 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Beta 1 (CTNNb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Beta 1 (CTNNb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Beta 1 (CTNNb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Beta 1 (CTNNb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Beta 1 (CTNNb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CTNNB1 Kits ELISA
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Autre désignation
    Catenin Beta 1 (CTNNB1 Produits)
    Synonymes
    Jup Kit ELISA, LOC100217813 Kit ELISA, CTNNB1 Kit ELISA, CTNNB Kit ELISA, Bfc Kit ELISA, Catnb Kit ELISA, Mesc Kit ELISA, ctnnb Kit ELISA, id:ibd2058 Kit ELISA, wu:fb73e10 Kit ELISA, wu:fi81c06 Kit ELISA, wu:fk25h01 Kit ELISA, ctnnb1 Kit ELISA, CHBCAT Kit ELISA, MRD19 Kit ELISA, armadillo Kit ELISA, beta-catenin Kit ELISA, catenin beta 1 Kit ELISA, catenin (cadherin associated protein), beta 1 Kit ELISA, catenin (cadherin-associated protein), beta 1 Kit ELISA, catenin beta 1 S homeolog Kit ELISA, catenin beta 1 L homeolog Kit ELISA, CTNNB1 Kit ELISA, Ctnnb1 Kit ELISA, ctnnb1 Kit ELISA, ctnnb1.S Kit ELISA, ctnnb1.L Kit ELISA
    Sujet

    Gene Name: Catenin Beta 1

    Gene Aliases: bCat, b-Cat, CTNN-B1, Cadherin-Associated Protein

    ID gène
    84353
    UniProt
    Q9WU82
    Pathways
    Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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