Cofilin Kit ELISA

N° du produit ABIN5653076

Détail du produit Cofilin Kit ELISA

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cofilin 1, Non Muscle (CFL1). No significant cross-reactivity or interference between Cofilin 1, Non Muscle (CFL1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cofilin 1, Non Muscle (CFL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cofilin 1, Non Muscle (CFL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cofilin 1, Non Muscle (CFL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cofilin 1, Non Muscle (CFL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cofilin 1, Non Muscle (CFL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: Cofilin 1, Non Muscle (CFL1 Produits)

Synonymes: cfl1 Kit ELISA, xac1 Kit ELISA, xac2 Kit ELISA, cfl Kit ELISA, cb86 Kit ELISA, sb:cb86 Kit ELISA, zgc:56501 Kit ELISA, wu:fb18a04 Kit ELISA, wu:fk77b03 Kit ELISA, 12/8 Kit ELISA, ADF Kit ELISA, ADF/cofilin Kit ELISA, Adf Kit ELISA, CG4254 Kit ELISA, Cadf Kit ELISA, D-cof Kit ELISA, D61 Kit ELISA, Dmel\\CG4254 Kit ELISA, T8 Kit ELISA, Tsr Kit ELISA, cofilin Kit ELISA, cofilin/ADF Kit ELISA, cofilin/actin depolymerizing factor homolog Kit ELISA, l(2)03101 Kit ELISA, l(2)k03101 Kit ELISA, l(2)k05633 Kit ELISA, l(2)k13409 Kit ELISA, ntf Kit ELISA, CFL Kit ELISA, AA959946 Kit ELISA, Cof Kit ELISA, CFL1 Kit ELISA, cofilin 1 (non-muscle) L homeolog Kit ELISA, cofilin 1 (non-muscle) Kit ELISA, cofilin 1 (non-muscle), like Kit ELISA, cofilin 1 Kit ELISA, twinstar Kit ELISA, cofilin Kit ELISA, cofilin 1, non-muscle Kit ELISA, Contrafreeloading Kit ELISA, cfl1.L Kit ELISA, cfl1 Kit ELISA, cfl1l Kit ELISA, CFL1 Kit ELISA, tsr Kit ELISA, COF1 Kit ELISA, TSTA_042790 Kit ELISA, Cfl1 Kit ELISA, CFL Kit ELISA

Sujet:

Gene Name: Cofilin 1, Non Muscle

Gene Aliases: CFL, p18, N-Cofilin, 18 kDa phosphoprotein

ID gène: 1072

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events