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Complement Factor I Kit ELISA

CFI Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
N° du produit ABIN5653279
  • Antigène Voir toutes Complement Factor I (CFI) Kits ELISA
    Complement Factor I (CFI)
    Reactivité
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Complement Factor I (CFI). No significant cross-reactivity or interference between Complement Factor I (CFI) and analogues was observed.
    Sensibilité
    0.072 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor I (CFI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor I (CFI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor I (CFI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor I (CFI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor I (CFI) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor I (CFI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Complement Factor I (CFI) Kits ELISA
    Complement Factor I (CFI)
    Autre désignation
    Complement Factor I (CFI Produits)
    Synonymes
    cfi Kit ELISA, MGC53615 Kit ELISA, Cfi Kit ELISA, factor I Kit ELISA, IF Kit ELISA, gb:ai721528 Kit ELISA, ahus3 Kit ELISA, c3b-ina Kit ELISA, c3bc4bi Kit ELISA, c3bina Kit ELISA, kaf Kit ELISA, CFI Kit ELISA, AHUS3 Kit ELISA, C3BINA Kit ELISA, C3b-INA Kit ELISA, FI Kit ELISA, KAF Kit ELISA, complement factor I S homeolog Kit ELISA, complement factor I L homeolog Kit ELISA, complement factor I Kit ELISA, complement component factor i Kit ELISA, cfi.S Kit ELISA, cfi.L Kit ELISA, CFI Kit ELISA, cfi Kit ELISA, Cfi Kit ELISA
    Sujet

    Gene Name: Complement Factor I

    Gene Aliases: CF-I, C3b-INA, FI, IF, KAF, Factor I, Konglutinogen-Activating Factor, C3b-Inactivator, C3B/C4B inactivator

    ID gène
    3426
    UniProt
    P05156
    Pathways
    Système du Complément
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