Connexin 43/GJA1 Kit ELISA

N° du produit ABIN5653304

Détail du produit Connexin 43/GJA1 Kit ELISA

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Connexin 43 (CX43). No significant cross-reactivity or interference between Connexin 43 (CX43) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 43 (CX43). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 43 (CX43). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 43 (CX43), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Connexin 43 (CX43) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 43 (CX43) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 43 (CX43) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Autre désignation: Connexin 43 (GJA1 Produits)

Synonymes: AVSD3 Kit ELISA, CX43 Kit ELISA, DFNB38 Kit ELISA, GJAL Kit ELISA, HLHS1 Kit ELISA, HSS Kit ELISA, ODDD Kit ELISA, Cx43 Kit ELISA, AU042049 Kit ELISA, AW546267 Kit ELISA, Cnx43 Kit ELISA, Cx43alpha1 Kit ELISA, Gja-1 Kit ELISA, Npm1 Kit ELISA, connexin43 Kit ELISA, cx43a1 Kit ELISA, gja1 Kit ELISA, zfCx43.3 Kit ELISA, connexin 43 Kit ELISA, gja1-A Kit ELISA, cx43 Kit ELISA, gjal Kit ELISA, oddd Kit ELISA, dfnb38 Kit ELISA, gap junction protein alpha 1 Kit ELISA, gap junction protein, alpha 1 Kit ELISA, connexin 43 Kit ELISA, gap junction protein alpha 1 L homeolog Kit ELISA, CONNEXIN 43 protein Kit ELISA, GJA1 Kit ELISA, Gja1 Kit ELISA, cx43 Kit ELISA, gja1.L Kit ELISA, gja1 Kit ELISA, CONNEXIN 43 Kit ELISA

Sujet:

Gene Name: Connexin 43

Gene Aliases: ODDD, GJAL, GJA1, GJ-A1, Gap junction 43 kDa heart protein, Gap Junction Protein Alpha 1, Oculodentodigital Dysplasia, Syndactyly Type III

ID gène: 14609

UniProt: P23242

Pathways: Signalisation MAPK, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization