Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CDK2 Kit ELISA

CDK2 Reactivité: Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
N° du produit ABIN5653420
  • Antigène Voir toutes CDK2 Kits ELISA
    CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
    Reactivité
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase 2 (CDK2). No significant cross-reactivity or interference between Cyclin Dependent Kinase 2 (CDK2) and analogues was observed.
    Sensibilité
    0.133 ng/mL
    Featured
    Discover our best selling CDK2 Kit ELISA
    Top Product
    Discover our top product CDK2 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase 2 (CDK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase 2 (CDK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase 2 (CDK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase 2 (CDK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase 2 (CDK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CDK2 Kits ELISA
    CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
    Autre désignation
    Cyclin Dependent Kinase 2 (CDK2 Produits)
    Synonymes
    p33(CDK2) Kit ELISA, A630093N05Rik Kit ELISA, wu:fa10c02 Kit ELISA, zgc:56598 Kit ELISA, zgc:77684 Kit ELISA, eg1 Kit ELISA, CDKN2 Kit ELISA, cdk2L Kit ELISA, cyclin dependent kinase 2 Kit ELISA, cyclin-dependent kinase 2 Kit ELISA, cyclin-dependent kinase 2 S homeolog Kit ELISA, CDK2 Kit ELISA, Cdk2 Kit ELISA, cdk2 Kit ELISA, cdk2.S Kit ELISA
    Sujet

    Gene Name: Cyclin Dependent Kinase 2

    Gene Aliases: CDKN2, p33(CDK2), Cell division protein kinase 2, p33 protein kinase

    UniProt
    Q63699
    Pathways
    Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
Vous êtes ici:
Support technique