Cytochrome C Kit ELISA

N° du produit ABIN5653488

Détail du produit Cytochrome C Kit ELISA

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivité: Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome C, Somatic (CYCS). No significant cross-reactivity or interference between Cytochrome C, Somatic (CYCS) and analogues was observed.

Sensibilité: 0.59 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C, Somatic (CYCS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C, Somatic (CYCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C, Somatic (CYCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C, Somatic (CYCS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C, Somatic (CYCS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C, Somatic (CYCS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Autre désignation: Cytochrome C, Somatic (CYCS Produits)

Synonymes: CYC Kit ELISA, HCS Kit ELISA, THC4 Kit ELISA, cyct Kit ELISA, cyc Kit ELISA, hcs Kit ELISA, CYCS Kit ELISA, cytc Kit ELISA, CYCSA Kit ELISA, CG17903 Kit ELISA, Cyt C Kit ELISA, Cyt c Kit ELISA, Cyt-c2 Kit ELISA, CytC-2 Kit ELISA, Cytc Kit ELISA, Cytc-p Kit ELISA, DC4 Kit ELISA, DMc01 Kit ELISA, Dmel\\CG17903 Kit ELISA, cyt c Kit ELISA, cyt c-p Kit ELISA, cyt-c-p Kit ELISA, cyt-c2 Kit ELISA, cyt.c Kit ELISA, cytochrome c Kit ELISA, dc4 Kit ELISA, pDMc01 Kit ELISA, wu:fk52b01 Kit ELISA, zgc:86706 Kit ELISA, ENSMUSG00000058927 Kit ELISA, cytochrome c, somatic Kit ELISA, cytochrome c, somatic S homeolog Kit ELISA, Cytochrome c proximal Kit ELISA, cytochrome c, somatic b Kit ELISA, cytochrome c, somatic pseudogene Kit ELISA, cytochrome c-like Kit ELISA, cytochrome c Kit ELISA, CYCS Kit ELISA, cycs.S Kit ELISA, cycs Kit ELISA, Cycs Kit ELISA, Cyt-c-p Kit ELISA, cycsb Kit ELISA, Gm10053 Kit ELISA, LOC100724240 Kit ELISA, CYC Kit ELISA, LOC100847700 Kit ELISA, LOC101107954 Kit ELISA, LOC106829744 Kit ELISA, LOC106992390 Kit ELISA, LOC102085712 Kit ELISA

Sujet:

Gene Name: Cytochrome C, Somatic

Gene Aliases: Cyt-C, HCS, CYC, CytC

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity