POR Kit ELISA

N° du produit ABIN5653523

Détail du produit POR Kit ELISA

Antigène: POR (P450 (Cytochrome) Oxidoreductase (POR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Reductase (CPR). No significant cross-reactivity or interference between Cytochrome P450 Reductase (CPR) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Reductase (CPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Reductase (CPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Reductase (CPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 Reductase (CPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Reductase (CPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: POR (P450 (Cytochrome) Oxidoreductase (POR))

Autre désignation: Cytochrome P450 Reductase (POR Produits)

Synonymes: CPR Kit ELISA, CYPOR Kit ELISA, P450R Kit ELISA, POR Kit ELISA, CCR Kit ELISA, CG11567 Kit ELISA, DMR Kit ELISA, DmCPR Kit ELISA, Dmel\\CG11567 Kit ELISA, NCPR Kit ELISA, P450 Kit ELISA, cpr Kit ELISA, zgc:110032 Kit ELISA, npr Kit ELISA, xpor Kit ELISA, 4933424M13Rik Kit ELISA, PORCINO Kit ELISA, T5J17.90 Kit ELISA, T5J17_90 Kit ELISA, TFC C Kit ELISA, TUBULIN-FOLDING COFACTOR C Kit ELISA, cytochrome p450 oxidoreductase Kit ELISA, P450 (cytochrome) oxidoreductase Kit ELISA, ADP-ribosylation factor interacting protein 2 Kit ELISA, Cytochrome P450 reductase Kit ELISA, P450 (cytochrome) oxidoreductase a Kit ELISA, P450 (cytochrome) oxidoreductase L homeolog Kit ELISA, NADPH--cytochrome P450 reductase Kit ELISA, C-CAP/cofactor C-like domain-containing protein Kit ELISA, POR Kit ELISA, Arfip2 Kit ELISA, Por Kit ELISA, Cpr Kit ELISA, pora Kit ELISA, por.L Kit ELISA, LOC100406157 Kit ELISA, por Kit ELISA, LOC100619047 Kit ELISA

Sujet:

Gene Name: Cytochrome P450 Reductase

Gene Aliases: POR, CYPOR, P450R, P450(Cytochrome)Oxidoreductase, NADPH--cytochrome P450 reductase

ID gène: 5447

UniProt: P16435

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, SARS-CoV-2 Protein Interactome