DKK1 Kit ELISA

N° du produit ABIN5653663

Détail du produit DKK1 Kit ELISA

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dickkopf Related Protein 1 (DKK1). No significant cross-reactivity or interference between Dickkopf Related Protein 1 (DKK1) and analogues was observed.

Sensibilité: 0.27 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 1 (DKK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 1 (DKK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 1 (DKK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dickkopf Related Protein 1 (DKK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dickkopf Related Protein 1 (DKK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: Dickkopf Related Protein 1 (DKK1 Produits)

Synonymes: DKK-1 Kit ELISA, SK Kit ELISA, AI414495 Kit ELISA, SGY Kit ELISA, SGY-1 Kit ELISA, SGY1 Kit ELISA, Soggy Kit ELISA, mSgy Kit ELISA, dkk1 Kit ELISA, dkk Kit ELISA, dkk-1 Kit ELISA, xdkk1 Kit ELISA, Xdkk-1 Kit ELISA, Dickkopf1 Kit ELISA, dickkopf-1 Kit ELISA, mdkk-1 Kit ELISA, dkk1-A Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 Kit ELISA, dickkopf like acrosomal protein 1 Kit ELISA, dickkopf-related protein 1 Kit ELISA, dickkopf-like 1 Kit ELISA, dickkopf WNT signaling pathway inhibitor 1b Kit ELISA, dickkopf protein Kit ELISA, dickkopf WNT signaling pathway inhibitor 1 S homeolog Kit ELISA, DKK1 Kit ELISA, Dkk1 Kit ELISA, DKKL1 Kit ELISA, LOC100228789 Kit ELISA, Dkkl1 Kit ELISA, dkk1b Kit ELISA, dkk1 Kit ELISA, dickkopf Kit ELISA, dkk1.S Kit ELISA

Sujet:

Gene Name: Dickkopf Related Protein 1

Gene Aliases: SK, Dickkopf Homolog 1(Xenopus Laevis)

ID gène: 108348170

UniProt: D3Z9J1

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation