DPYD Kit ELISA

N° du produit ABIN5653674

Détail du produit DPYD Kit ELISA

Antigène: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 1000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dihydropyrimidine Dehydrogenase (DPYD). No significant cross-reactivity or interference between Dihydropyrimidine Dehydrogenase (DPYD) and analogues was observed.

Sensibilité: 26.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dihydropyrimidine Dehydrogenase (DPYD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dihydropyrimidine Dehydrogenase (DPYD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dihydropyrimidine Dehydrogenase (DPYD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dihydropyrimidine Dehydrogenase (DPYD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Autre désignation: Dihydropyrimidine Dehydrogenase (DPYD Produits)

Synonymes: DPYD Kit ELISA, dpyd Kit ELISA, zgc:77205 Kit ELISA, AI315208 Kit ELISA, DPD Kit ELISA, E330028L06Rik Kit ELISA, DHP Kit ELISA, DHPDHASE Kit ELISA, dhp Kit ELISA, dhpdhase Kit ELISA, dpd Kit ELISA, 14.t00017 Kit ELISA, DDBDRAFT_0189681 Kit ELISA, DDBDRAFT_0231100 Kit ELISA, DDB_0189681 Kit ELISA, DDB_0231100 Kit ELISA, dpyda Kit ELISA, zgc:153505 Kit ELISA, dihydropyrimidine dehydrogenase Kit ELISA, dihydropyrimidine dehydrogenase b Kit ELISA, dihydropyrimidine dehydrogenase L homeolog Kit ELISA, dihydropyrimidine dehydrogenase [NADP(+)] Kit ELISA, dihydropyrimidine dehydrogenase a, tandem duplicate 1 Kit ELISA, DPYD Kit ELISA, Dpyd Kit ELISA, dpydb Kit ELISA, dpyd.L Kit ELISA, EHI_012980 Kit ELISA, LOC5573038 Kit ELISA, EDI_044620 Kit ELISA, pyd1 Kit ELISA, dpyda.1 Kit ELISA

Sujet:

Gene Name: Dihydropyrimidine Dehydrogenase

Gene Aliases: DPD, DHPDHase, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase

ID gène: 1806

UniProt: Q12882

Pathways: Ribonucleoside Biosynthetic Process