DNMT1 Kit ELISA

N° du produit ABIN5653707

Détail du produit DNMT1 Kit ELISA

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of DNA Methyltransferase 1 (DNMT1). No significant cross-reactivity or interference between DNA Methyltransferase 1 (DNMT1) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Methyltransferase 1 (DNMT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Methyltransferase 1 (DNMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Methyltransferase 1 (DNMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Methyltransferase 1 (DNMT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DNA Methyltransferase 1 (DNMT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNA Methyltransferase 1 (DNMT1 Produits)

Synonymes: cb1075 Kit ELISA, cb983 Kit ELISA, dmnt1 Kit ELISA, fb05h01 Kit ELISA, mta Kit ELISA, wu:fb05h01 Kit ELISA, ADCADN Kit ELISA, AIM Kit ELISA, CXXC9 Kit ELISA, DNMT Kit ELISA, HSN1E Kit ELISA, MCMT Kit ELISA, DDM2 Kit ELISA, DECREASED DNA METHYLATION 2 Kit ELISA, DMT01 Kit ELISA, DMT1 Kit ELISA, DNA METHYLTRANSFERASE Kit ELISA, DNA METHYLTRANSFERASE 01 Kit ELISA, DNA METHYLTRANSFERASE 1 Kit ELISA, MET2 Kit ELISA, METHYLTRANSFERASE 2 Kit ELISA, METHYLTRANSFERASE I Kit ELISA, METI Kit ELISA, methyltransferase 1 Kit ELISA, Cxxc9 Kit ELISA, Dnmt Kit ELISA, Dnmt1o Kit ELISA, MTase Kit ELISA, Met-1 Kit ELISA, Met1 Kit ELISA, MommeD2 Kit ELISA, m.MmuI Kit ELISA, DNA methyltransferase 1 Kit ELISA, DNA (cytosine-5-)-methyltransferase 1 Kit ELISA, methyltransferase 1 Kit ELISA, DNA methyltransferase (cytosine-5) 1 Kit ELISA, DNMT1 Kit ELISA, dnmt1 Kit ELISA, Dnmt1 Kit ELISA, MET1 Kit ELISA

Sujet:

Gene Name: DNA Methyltransferase 1

Gene Aliases: CXXC9, DNMT, MCMT, Cytosine-5 DNA Methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2