Dopamine d2 Receptor Kit ELISA
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- Antigène Voir toutes Dopamine d2 Receptor (DRD2) Kits ELISA
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Dopamine Receptor D2 (DRD2). No significant cross-reactivity or interference between Dopamine Receptor D2 (DRD2) and analogues was observed.
- Sensibilité
- 0.123 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Receptor D2 (DRD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Receptor D2 (DRD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Receptor D2 (DRD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine Receptor D2 (DRD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Receptor D2 (DRD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Receptor D2 (DRD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Dopamine d2 Receptor (DRD2) Kits ELISA
- Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))
- Autre désignation
- Dopamine Receptor D2 (DRD2 Produits)
- Synonymes
- D2DR Kit ELISA, D2R Kit ELISA, drd2b Kit ELISA, d2r2 Kit ELISA, Drd-2 Kit ELISA, d2dr Kit ELISA, d2r Kit ELISA, drd2 Kit ELISA, drd2-B Kit ELISA, DRD2 Kit ELISA, D2 Kit ELISA, CG18741 Kit ELISA, CG7569 Kit ELISA, DAMB Kit ELISA, DDR2 Kit ELISA, DOPR2 Kit ELISA, DOPR99B Kit ELISA, Dar2 Kit ELISA, Dmel\\CG18741 Kit ELISA, DopD1 Kit ELISA, DopR99B Kit ELISA, DopRII Kit ELISA, dopamine receptor D2 Kit ELISA, dopamine receptor D2 like Kit ELISA, dopamine receptor D2 L homeolog Kit ELISA, dopamine receptor D2 S homeolog Kit ELISA, Dopamine 1-like receptor 2 Kit ELISA, DRD2 Kit ELISA, drd2l Kit ELISA, drd2 Kit ELISA, Drd2 Kit ELISA, drd2.L Kit ELISA, drd2.S Kit ELISA, Dop1R2 Kit ELISA
- Sujet
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Gene Name: Dopamine Receptor D2
Gene Aliases: DR-D2, D2DR, D2R
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity
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