DYNC1H1 Kit ELISA

N° du produit ABIN5653757

Détail du produit DYNC1H1 Kit ELISA

Antigène: DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). No significant cross-reactivity or interference between Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DYNC1H1 (Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1))

Autre désignation: Dynein, Cytoplasmic 1, Heavy Chain 1 (DYNC1H1 Produits)

Synonymes: fk70a07 Kit ELISA, wu:fk70a07 Kit ELISA, p22 Kit ELISA, dhc1 Kit ELISA, dncl Kit ELISA, dyhc Kit ELISA, hl-3 Kit ELISA, dhc1a Kit ELISA, dnch1 Kit ELISA, dnecl Kit ELISA, dnchc1 Kit ELISA, Dnch1 Kit ELISA, Dnchc1 Kit ELISA, 9930018I23Rik Kit ELISA, AI894280 Kit ELISA, DHC1 Kit ELISA, DHC1a Kit ELISA, DNCL Kit ELISA, Dnec1 Kit ELISA, Dnecl Kit ELISA, Loa Kit ELISA, MAP1C Kit ELISA, P22 Kit ELISA, Swl Kit ELISA, mKIAA0325 Kit ELISA, DNCH1 Kit ELISA, DNECL Kit ELISA, DYHC Kit ELISA, HL-3 Kit ELISA, SMALED1 Kit ELISA, dynein, cytoplasmic 1, heavy chain 1 Kit ELISA, dynein cytoplasmic 1 heavy chain 1 Kit ELISA, dync1h1 Kit ELISA, DYNC1H1 Kit ELISA, Dync1h1 Kit ELISA

Sujet:

Gene Name: Dynein, Cytoplasmic 1, Heavy Chain 1

Gene Aliases: DHC1, DHC1a, DNCH1, DNCL, DNECL, DYHC, Dnchc1, HL-3, p22, Dynein heavy chain, cytosolic, Cytoplasmic dynein heavy chain 1, Cytoplasmic dynein 1 heavy chain 1

ID gène: 1778

UniProt: Q14204

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization