Dystroglycan Kit ELISA

N° du produit ABIN5653764

Détail du produit Dystroglycan Kit ELISA

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Dystrophin Associated Glycoprotein 1 (DAG1). No significant cross-reactivity or interference between Dystrophin Associated Glycoprotein 1 (DAG1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dystrophin Associated Glycoprotein 1 (DAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dystrophin Associated Glycoprotein 1 (DAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dystrophin Associated Glycoprotein 1 (DAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dystrophin Associated Glycoprotein 1 (DAG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Autre désignation: Dystrophin Associated Glycoprotein 1 (DAG1 Produits)

Synonymes: LOC398500 Kit ELISA, dag1 Kit ELISA, MGC53537 Kit ELISA, 156DAG Kit ELISA, A3a Kit ELISA, AGRNR Kit ELISA, DAG Kit ELISA, MDDGC7 Kit ELISA, MDDGC9 Kit ELISA, DAG1 Kit ELISA, RAB7 Kit ELISA, dg Kit ELISA, a3a Kit ELISA, dag Kit ELISA, agrnr Kit ELISA, 156dag Kit ELISA, D9Wsu13e Kit ELISA, DG Kit ELISA, Dp427 Kit ELISA, Dp71 Kit ELISA, CG18250 Kit ELISA, CT41273 Kit ELISA, DmDG Kit ELISA, Dmel\\CG18250 Kit ELISA, atu Kit ELISA, dgn Kit ELISA, dys Kit ELISA, GB14967 Kit ELISA, APOJ Kit ELISA, CLI Kit ELISA, RATTRPM2B Kit ELISA, SGP-2 Kit ELISA, SGP2 Kit ELISA, SP-40 Kit ELISA, SP40 Kit ELISA, TRPM-2 Kit ELISA, TRPM2B Kit ELISA, Trpm2 Kit ELISA, Trpmb Kit ELISA, Ala-1 Kit ELISA, H9/25 Kit ELISA, Ly-27 Kit ELISA, Ly-6 Kit ELISA, Ly27 Kit ELISA, wu:fb83d06 Kit ELISA, wu:fi25f06 Kit ELISA, wu:fi37b08 Kit ELISA, zgc:109786 Kit ELISA, DystroGlycaN Kit ELISA, dystroglycan 1 L homeolog Kit ELISA, dystroglycan 1 Kit ELISA, dystroglycan 1 S homeolog Kit ELISA, Dystroglycan Kit ELISA, dystroglycan Kit ELISA, clusterin Kit ELISA, lymphocyte antigen 6 complex Kit ELISA, dgn-1 Kit ELISA, dag1.L Kit ELISA, dgn-2 Kit ELISA, dgn-3 Kit ELISA, DAG1 Kit ELISA, dag1.S Kit ELISA, Dag1 Kit ELISA, dag1 Kit ELISA, Dg Kit ELISA, LOC408826 Kit ELISA, Clu Kit ELISA, Ly6 Kit ELISA

Sujet:

Gene Name: Dystrophin Associated Glycoprotein 1

Gene Aliases: Dystroglycan 1, 156DAG, A3a, AGRNR, DAG, Alpha-Dystroglycan, Beta-Dystroglycan

ID gène: 13138

UniProt: Q62165

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus