EGR1 Kit ELISA

N° du produit ABIN5653772

Détail du produit EGR1 Kit ELISA

Antigène: EGR1 (Early Growth Response 1 (EGR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Early Growth Response Protein 1 (EGR1). No significant cross-reactivity or interference between Early Growth Response Protein 1 (EGR1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Early Growth Response Protein 1 (EGR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Early Growth Response Protein 1 (EGR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Early Growth Response Protein 1 (EGR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Early Growth Response Protein 1 (EGR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Early Growth Response Protein 1 (EGR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EGR1 (Early Growth Response 1 (EGR1))

Autre désignation: Early Growth Response Protein 1 (EGR1 Produits)

Synonymes: AT225 Kit ELISA, G0S30 Kit ELISA, KROX-24 Kit ELISA, NGFI-A Kit ELISA, TIS8 Kit ELISA, ZIF-268 Kit ELISA, ZNF225 Kit ELISA, A530045N19Rik Kit ELISA, ETR103 Kit ELISA, Egr-1 Kit ELISA, Krox-1 Kit ELISA, Krox-24 Kit ELISA, Krox24 Kit ELISA, NGF1-A Kit ELISA, NGFIA Kit ELISA, Zenk Kit ELISA, Zfp-6 Kit ELISA, Zif268 Kit ELISA, egr Kit ELISA, Ngf1 Kit ELISA, Ngfi Kit ELISA, zif-268 Kit ELISA, EGR1 Kit ELISA, EGR-1-A Kit ELISA, Xegr-1 Kit ELISA, at225 Kit ELISA, egr-1 Kit ELISA, egr1 Kit ELISA, g0s30 Kit ELISA, krox-24 Kit ELISA, ngfi-a Kit ELISA, tis8 Kit ELISA, znf225 Kit ELISA, krox24 Kit ELISA, wu:fj64b05 Kit ELISA, wu:fq25f01 Kit ELISA, EGR-1 Kit ELISA, zenk Kit ELISA, EGR-1-B Kit ELISA, egr1-a Kit ELISA, egr1-b Kit ELISA, early growth response 1 Kit ELISA, early growth response protein 1 (egr-1) Kit ELISA, early growth response 1 L homeolog Kit ELISA, early growth response 1 S homeolog Kit ELISA, EGR1 Kit ELISA, CNK00890 Kit ELISA, Egr1 Kit ELISA, egr1.L Kit ELISA, egr1 Kit ELISA, egr1.S Kit ELISA

Sujet:

Gene Name: Early Growth Response Protein 1

Gene Aliases: Zif268, AT225, G0S30, KROX24, NGFI-A, TIS8, ZIF-268, ZNF225, NGFIA, TIS8, ZENK, Nerve Growth Factor-Induced Protein A, Transcription Factor ETR103, Zinc Finger Protein 225

ID gène: 1958

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity