EGLN1 Kit ELISA

N° du produit ABIN5653787

Détail du produit EGLN1 Kit ELISA

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Egl Nine Homolog 1 (EGLN1). No significant cross-reactivity or interference between Egl Nine Homolog 1 (EGLN1) and analogues was observed.

Sensibilité: 13.5 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1 (EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1 (EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1 (EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Egl Nine Homolog 1 (EGLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Egl Nine Homolog 1 (EGLN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Egl Nine Homolog 1 (EGLN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Autre désignation: Egl Nine Homolog 1 (EGLN1 Produits)

Synonymes: EGLN1 Kit ELISA, egln2 Kit ELISA, phd2 Kit ELISA, zgc:92314 Kit ELISA, AI503754 Kit ELISA, C1orf12 Kit ELISA, HIF-PH2 Kit ELISA, HPH-2 Kit ELISA, Hif-p4h-2 Kit ELISA, ORF13 Kit ELISA, Phd2 Kit ELISA, SM-20 Kit ELISA, PHD-2 Kit ELISA, PHD2 Kit ELISA, ECYT3 Kit ELISA, HIFPH2 Kit ELISA, HPH2 Kit ELISA, SM20 Kit ELISA, ZMYND6 Kit ELISA, egl nine homolog 1 (C. elegans) Kit ELISA, egl-9 family hypoxia inducible factor 1 Kit ELISA, egl-9 family hypoxia-inducible factor 1 S homeolog Kit ELISA, egl-9 family hypoxia-inducible factor 1b Kit ELISA, egl-9 family hypoxia-inducible factor 1 Kit ELISA, EGLN1 Kit ELISA, egln1.S Kit ELISA, egln1b Kit ELISA, Egln1 Kit ELISA

Sujet:

Gene Name: Egl Nine Homolog 1

Gene Aliases: ECYT3, HIFPH2, PHD2, C1orf12, SM20, ZMYND6, HIF Prolyl Hydroxylase 2, Prolyl hydroxylase domain-containing protein 2, Hypoxia-inducible factor prolyl hydroxylase 2

Pathways: cAMP Metabolic Process, L'effet Warburg