eEF1A1 Kit ELISA

N° du produit ABIN5654003

Détail du produit eEF1A1 Kit ELISA

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). No significant cross-reactivity or interference between Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Autre désignation: Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1A1 Produits)

Synonymes: CCS-3 Kit ELISA, CCS3 Kit ELISA, EE1A1 Kit ELISA, EEF-1 Kit ELISA, EEF1A Kit ELISA, EF-Tu Kit ELISA, EF1A Kit ELISA, GRAF-1EF Kit ELISA, HNGC:16303 Kit ELISA, LENG7 Kit ELISA, PTI1 Kit ELISA, eEF1A-1 Kit ELISA, Eef1a2 Kit ELISA, Eef1a2l1 Kit ELISA, SI Kit ELISA, EEF1A2 Kit ELISA, EF1A1 Kit ELISA, eef1a1 Kit ELISA, wu:fj34g08 Kit ELISA, zgc:110335 Kit ELISA, EEF1A1 Kit ELISA, RABEFLA2 Kit ELISA, EFL1-alpha Kit ELISA, chunp6927 Kit ELISA, eef1a Kit ELISA, ef1a Kit ELISA, ik:tdsubc_2a3 Kit ELISA, ik:tdsubc_2b3 Kit ELISA, tdsubc_2a3 Kit ELISA, wu:fa91c07 Kit ELISA, wu:fa94b03 Kit ELISA, wu:fi13b09 Kit ELISA, xx:tdsubc_2a3 Kit ELISA, xx:tdsubc_2b3 Kit ELISA, EF-1A Kit ELISA, EF-1-ALPHA-S Kit ELISA, eef1a-s Kit ELISA, eef1as Kit ELISA, fj64c02 Kit ELISA, wu:fj64c02 Kit ELISA, zgc:73138 Kit ELISA, eukaryotic translation elongation factor 1 alpha 1 Kit ELISA, eukaryotic translation elongation factor 1 alpha 1b Kit ELISA, eukaryotic translation elongation factor 1 alpha 1, like 1 Kit ELISA, eukaryotic translation elongation factor 1 alpha 1 L homeolog Kit ELISA, elongation factor 1-alpha Kit ELISA, eukaryotic translation elongation factor 1 alpha 1a Kit ELISA, EEF1A1 Kit ELISA, Eef1a1 Kit ELISA, eef1a1b Kit ELISA, eef1a1l1 Kit ELISA, eef1a1.L Kit ELISA, tufA Kit ELISA, eef1a1a Kit ELISA

Sujet:

Gene Name: Eukaryotic Translation Elongation Factor 1 Alpha 1

Gene Aliases: CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, LENG7, PTI1, eEF1A-1, Leukocyte Receptor Cluster(LRC)Member 7, Elongation factor Tu

ID gène: 1915

UniProt: P68104