ERCC1 Kit ELISA

N° du produit ABIN5654008

Détail du produit ERCC1 Kit ELISA

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). No significant cross-reactivity or interference between Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Autre désignation: Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 (ERCC1 Produits)

Synonymes: COFS4 Kit ELISA, RAD10 Kit ELISA, UV20 Kit ELISA, Ercc-1 Kit ELISA, ercc1 Kit ELISA, T12H1.18 Kit ELISA, T12H1_18 Kit ELISA, UV REPAIR DEFICIENT 7 Kit ELISA, UVR7 Kit ELISA, ERCC excision repair 1, endonuclease non-catalytic subunit Kit ELISA, excision repair cross-complementing rodent repair deficiency, complementation group 1 Kit ELISA, DNA excision repair protein ERCC-1 Kit ELISA, nucleotide repair protein Kit ELISA, excision repair cross-complementation group 1 L homeolog Kit ELISA, excision repair cross-complementation group 1 Kit ELISA, ERCC1 Kit ELISA, Ercc1 Kit ELISA, CC1G_04546 Kit ELISA, CpipJ_CPIJ013903 Kit ELISA, PITG_13719 Kit ELISA, ercc1 Kit ELISA, ercc1.L Kit ELISA

Sujet:

Gene Name: Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1

Gene Aliases: UV20, DNA excision repair protein ERCC-1

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response