Filamin A Kit ELISA
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- Antigène Voir toutes Filamin A (FLNA) Kits ELISA
- Filamin A (FLNA) (Filamin A, alpha (FLNA))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 1.88 ng/mL - 120 ng/mL
- Seuil minimal de détection
- 1.88 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Filamin A Alpha (FLNa). No significant cross-reactivity or interference between Filamin A Alpha (FLNa) and analogues was observed.
- Sensibilité
- 0.65 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha (FLNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin A Alpha (FLNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin A Alpha (FLNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin A Alpha (FLNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin A Alpha (FLNa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Filamin A (FLNA) Kits ELISA
- Filamin A (FLNA) (Filamin A, alpha (FLNA))
- Autre désignation
- Filamin A Alpha (FLNA Produits)
- Synonymes
- ABP-280 Kit ELISA, ABPX Kit ELISA, CSBS Kit ELISA, CVD1 Kit ELISA, FLN Kit ELISA, FLN-A Kit ELISA, FLN1 Kit ELISA, FMD Kit ELISA, MNS Kit ELISA, NHBP Kit ELISA, OPD Kit ELISA, OPD1 Kit ELISA, OPD2 Kit ELISA, XLVD Kit ELISA, XMVD Kit ELISA, fb98b06 Kit ELISA, wu:fb98b06 Kit ELISA, wu:fd09g03 Kit ELISA, wu:fd49a06 Kit ELISA, Dilp2 Kit ELISA, F730004A14Rik Kit ELISA, Fln1 Kit ELISA, filamin-1 Kit ELISA, RGD1560614 Kit ELISA, MGC147094 Kit ELISA, CG3937 Kit ELISA, Cher Kit ELISA, Dmel\\CG3937 Kit ELISA, Fil Kit ELISA, joy Kit ELISA, sko Kit ELISA, filamin A Kit ELISA, filamin A, alpha (actin binding protein 280) Kit ELISA, filamin, alpha Kit ELISA, filamin-A Kit ELISA, cheerio Kit ELISA, FLNA Kit ELISA, flna Kit ELISA, Flna Kit ELISA, LOC587317 Kit ELISA, cher Kit ELISA, LOC100380643 Kit ELISA
- Sujet
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Gene Name: Filamin A Alpha
Gene Aliases: ABP280, FLN-A, ABPX, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, Actin Binding Protein 280, Endothelial actin-binding protein, Filamin-1, Non-muscle filamin
- ID gène
- 2316
- UniProt
- P21333
- Pathways
- TCR Signaling, Maintenance of Protein Location
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