GLUD1 Kit ELISA

N° du produit ABIN5654653

Détail du produit GLUD1 Kit ELISA

Antigène: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutamate Dehydrogenase 1 (GDH). No significant cross-reactivity or interference between Glutamate Dehydrogenase 1 (GDH) and analogues was observed.

Sensibilité: 0.068 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Dehydrogenase 1 (GDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Dehydrogenase 1 (GDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Autre désignation: Glutamate Dehydrogenase 1 (GLUD1 Produits)

Synonymes: GDH Kit ELISA, gdh1 Kit ELISA, GDH 1 Kit ELISA, glud1 Kit ELISA, GLUD1 Kit ELISA, cb719 Kit ELISA, wu:fb16e02 Kit ELISA, wu:fb58f12 Kit ELISA, wu:fe37f03 Kit ELISA, wu:fj43f02 Kit ELISA, zgc:192851 Kit ELISA, zgc:55630 Kit ELISA, GDH1 Kit ELISA, GLUD Kit ELISA, AI118167 Kit ELISA, Gdh-X Kit ELISA, Glud Kit ELISA, Gludl Kit ELISA, Ac2-281 Kit ELISA, Gdh1 Kit ELISA, Gludeha Kit ELISA, MRG-2 Kit ELISA, GLUTAMATE DECARBOXYLASE 1 Kit ELISA, GLUTAMATE DEHYDROGENASE 1 Kit ELISA, MRG7.13 Kit ELISA, MRG7_13 Kit ELISA, glutamate dehydrogenase 1 Kit ELISA, C2H1orf130 Kit ELISA, legdh1 Kit ELISA, cb622 Kit ELISA, wu:fc33g09 Kit ELISA, wu:fc66a10 Kit ELISA, zgc:77186 Kit ELISA, glutamate dehydrogenase 1 Kit ELISA, glutamate dehydrogenase 1 S homeolog Kit ELISA, glutamate dehydrogenase 1, mitochondrial Kit ELISA, glutamate dehydrogenase 1b Kit ELISA, glutamate dehydrogenase Kit ELISA, non-compact myelin associated protein Kit ELISA, glutamate dehydrogenase 1a Kit ELISA, GLUD1 Kit ELISA, GDH1 Kit ELISA, glud1 Kit ELISA, glud1.S Kit ELISA, LOC693461 Kit ELISA, glud1b Kit ELISA, Glud1 Kit ELISA, HACJB3_RS00320 Kit ELISA, LOC100587725 Kit ELISA, NCMAP Kit ELISA, gdh1 Kit ELISA, glud1a Kit ELISA

Sujet:

Gene Name: Glutamate Dehydrogenase 1

Gene Aliases: GLUD1, Glutamate dehydrogenase 1, mitochondrial

ID gène: 2746

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, L'effet Warburg