Glutaminase Kit ELISA

N° du produit ABIN5654676

Détail du produit Glutaminase Kit ELISA

Antigène: Glutaminase (GLS)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutaminase (GLS). No significant cross-reactivity or interference between Glutaminase (GLS) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaminase (GLS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaminase (GLS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaminase (GLS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutaminase (GLS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutaminase (GLS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutaminase (GLS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glutaminase (GLS)

Autre désignation: Glutaminase (GLS Produits)

Synonymes: Glut Kit ELISA, RATGLUT Kit ELISA, gls Kit ELISA, si:dz87i4.2 Kit ELISA, wu:fa97e05 Kit ELISA, GA Kit ELISA, PAG Kit ELISA, AAD20 Kit ELISA, GAC Kit ELISA, GAM Kit ELISA, GLS1 Kit ELISA, KGA Kit ELISA, AI314027 Kit ELISA, 6330442B14 Kit ELISA, B230365M23Rik Kit ELISA, CG8772 Kit ELISA, CG8872 Kit ELISA, Dmel\\CG42708 Kit ELISA, Dmel_CG8772 Kit ELISA, BA3155 Kit ELISA, glutaminase Kit ELISA, glutaminase b Kit ELISA, Glutaminase Kit ELISA, glutaminase kidney isoform, mitochondrial Kit ELISA, Gls Kit ELISA, GLS Kit ELISA, gls Kit ELISA, glsb Kit ELISA, glsA2 Kit ELISA, glsA-2 Kit ELISA, Arnit_3113 Kit ELISA, Celal_2913 Kit ELISA, Celly_0289 Kit ELISA, Weevi_2101 Kit ELISA, Mesop_4683 Kit ELISA, LOC100069617 Kit ELISA

Sujet:

Gene Name: Glutaminase

Gene Aliases: GLS1, Glutaminase kidney isoform, mitochondrial, K-glutaminase, L-glutamine amidohydrolase

ID gène: 2744

UniProt: O94925

Pathways: Feeding Behaviour, Dicarboxylic Acid Transport, L'effet Warburg