Glutathione Peroxidase 1 Kit ELISA

N° du produit ABIN5654694

Détail du produit Glutathione Peroxidase 1 Kit ELISA

Antigène: Glutathione Peroxidase 1 (GPX1)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 1 (GPX1). No significant cross-reactivity or interference between Glutathione Peroxidase 1 (GPX1) and analogues was observed.

Sensibilité: 0.132 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 1 (GPX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Glutathione Peroxidase 1 (GPX1)

Autre désignation: Glutathione Peroxidase 1 (GPX1 Produits)

Synonymes: Gpx Kit ELISA, ATGPX1 Kit ELISA, F13D4.40 Kit ELISA, F13D4_40 Kit ELISA, GLUTATHIONE PEROXIDASE 1 Kit ELISA, glutathione peroxidase 1 Kit ELISA, gshpx1 Kit ELISA, AI195024 Kit ELISA, AL033363 Kit ELISA, CGPx Kit ELISA, GPx-1 Kit ELISA, GSHPx-1 Kit ELISA, GPX1 Kit ELISA, gpx1 Kit ELISA, cb690 Kit ELISA, zgc:92737 Kit ELISA, zgc:103683 Kit ELISA, GPXD Kit ELISA, GSHPX1 Kit ELISA, GSHPx Kit ELISA, wu:fd42f12 Kit ELISA, wu:fj22d06 Kit ELISA, zgc:101700 Kit ELISA, glutathione peroxidase Gpx1 Kit ELISA, glutathione peroxidase Kit ELISA, glutathione peroxidase 1 Kit ELISA, glutathione peroxidase 1a Kit ELISA, glutathione peroxidase family protein Kit ELISA, proliferating cell nuclear antigen Kit ELISA, glutathione peroxidase 1b Kit ELISA, glutathione peroxidase 1 S homeolog Kit ELISA, gpx1 Kit ELISA, GPX1 Kit ELISA, gpx Kit ELISA, Gpx Kit ELISA, Gpx1 Kit ELISA, gpx1a Kit ELISA, POPTR_0003s12620g Kit ELISA, Pcna Kit ELISA, LBL_RS00590 Kit ELISA, btuE-1 Kit ELISA, GPX2 Kit ELISA, gpx1b Kit ELISA, gpx1.S Kit ELISA

Sujet:

Gene Name: Glutathione Peroxidase 1

Gene Aliases: GSHPX1, Cellular glutathione peroxidase

Pathways: Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome