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GPLD1 Kit ELISA

GPLD1 Reactivité: Humain Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum
N° du produit ABIN5654773
  • Antigène Voir toutes GPLD1 Kits ELISA
    GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))
    Reactivité
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.781 ng/mL - 50 ng/mL
    Seuil minimal de détection
    0.781 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1). No significant cross-reactivity or interference between Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) and analogues was observed.
    Sensibilité
    0.34 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes GPLD1 Kits ELISA
    GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))
    Autre désignation
    Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1 Produits)
    Synonymes
    GPIPLD Kit ELISA, GPIPLDM Kit ELISA, PIGPLD Kit ELISA, PIGPLD1 Kit ELISA, 6330541J12Rik Kit ELISA, AW546131 Kit ELISA, glycosylphosphatidylinositol specific phospholipase D1 Kit ELISA, glycosylphosphatidylinositol specific phospholipase d1 Kit ELISA, GPLD1 Kit ELISA, gpld1 Kit ELISA, Micau_0957 Kit ELISA, ML5_1205 Kit ELISA, VAB18032_09490 Kit ELISA, Gpld1 Kit ELISA
    Sujet

    Gene Name: Glycosylphosphatidylinositol Specific Phospholipase D1

    Gene Aliases: GPIPLD, GPIPLDM, PIGPLD, PIGPLD1, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D

    ID gène
    2822
    UniProt
    P80108
    Pathways
    Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process
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