GAS6 Kit ELISA

N° du produit ABIN5654831

Détail du produit GAS6 Kit ELISA

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Growth Arrest Specific Protein 6 (GAS6). No significant cross-reactivity or interference between Growth Arrest Specific Protein 6 (GAS6) and analogues was observed.

Sensibilité: 33 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest Specific Protein 6 (GAS6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest Specific Protein 6 (GAS6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest Specific Protein 6 (GAS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest Specific Protein 6 (GAS6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Arrest Specific Protein 6 (GAS6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Arrest Specific Protein 6 (GAS6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Autre désignation: Growth Arrest Specific Protein 6 (GAS6 Produits)

Synonymes: fj34a12 Kit ELISA, zgc:63860 Kit ELISA, wu:fj34a12 Kit ELISA, MGC68463 Kit ELISA, gas6 Kit ELISA, MGC83872 Kit ELISA, GAS6 Kit ELISA, Gas-6 Kit ELISA, AXLLG Kit ELISA, AXSF Kit ELISA, growth arrest specific 6 Kit ELISA, growth arrest-specific 6 Kit ELISA, growth arrest specific 6 L homeolog Kit ELISA, growth arrest specific 6 S homeolog Kit ELISA, GAS6 Kit ELISA, gas6 Kit ELISA, gas6.L Kit ELISA, gas6.S Kit ELISA, Gas6 Kit ELISA

Sujet:

Gene Name: Growth Arrest Specific Protein 6

Gene Aliases: AXLLG, AXSF, AXL Stimulatory Factor, AXL receptor tyrosine kinase ligand

UniProt: Q63772

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response