GRB10 Kit CLIA

N° du produit ABIN5654875

Détail du produit GRB10 Kit CLIA

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 312.5 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312.5 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 10 (Grb10). No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 10 (Grb10) and analogues was observed.

Sensibilité: 10.02 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 10 (Grb10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Factor Receptor Bound Protein 10 (Grb10) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Autre désignation: Growth Factor Receptor Bound Protein 10 (GRB10 Produits)

Synonymes: grb10 Kit CLIA, zgc:91987 Kit CLIA, GRB10 Kit CLIA, grb-10 Kit CLIA, grb-ir Kit CLIA, irbp Kit CLIA, meg1 Kit CLIA, rss Kit CLIA, LOC100224775 Kit CLIA, 5730571D09Rik Kit CLIA, AI325020 Kit CLIA, Meg1 Kit CLIA, mKIAA0207 Kit CLIA, RGD1566234 Kit CLIA, GRB-IR Kit CLIA, Grb-10 Kit CLIA, IRBP Kit CLIA, MEG1 Kit CLIA, RSS Kit CLIA, growth factor receptor bound protein 10 Kit CLIA, growth factor receptor-bound protein 10a Kit CLIA, growth factor receptor bound protein 10 L homeolog Kit CLIA, growth factor receptor-bound protein 10 Kit CLIA, GRB10 Kit CLIA, grb10a Kit CLIA, grb10 Kit CLIA, grb10.L Kit CLIA, LOC100434903 Kit CLIA, Grb10 Kit CLIA

Sujet:

Gene Name: Growth Factor Receptor Bound Protein 10

Gene Aliases: GRB-IR, IRBP, MEG1, RSS, GRB10 adapter protein, Insulin receptor-binding protein Grb-IR

ID gène: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2